Depleting Gene Activities in Early Drosophila Embryos with the “Maternal-Gal4–shRNA” System

摘要: In a developing Drosophila melanogaster embryo, mRNAs have maternal origin, zygotic or both. During the maternal–zygotic transition, products are degraded and gene expression comes under control of genome. To interrogate function that both maternally zygotically expressed, it is common to examine embryonic phenotypes derived from female germline mosaics. Recently, development RNAi vectors based on short hairpin RNAs (shRNAs) effective during oogenesis has provided an alternative producing clones. Here, we evaluate efficacies of: (1) loaded shRNAs knockdown transcripts (2) Gal4 protein drive shRNA expression. We show that, while Gal4-driven in very effectively generate for genes expressed maternally, not at generating early genes. However, efficient mid-embryogenesis. apply this powerful simple method unravel functions number pleiotropic