Diet-dependent genetic and genomic imprinting effects on obesity in mice.

摘要: Although the current obesity epidemic is of environmental origin, there substantial genetic variation in individual response to an obesogenic environment. In this study, we perform a genome-wide scan for quantitative trait loci (QTLs) affecting per se, or obese high-fat diet mice from LG/J by SM/J Advanced Intercross (AI) Line (Wustl:LG,SM-G16). A total 1,002 animals 78 F₁₆ full sibships were weaned at 3 weeks age and half each litter placed on high- low-fat diets. Animals remained until 20 when they necropsied weights reproductive, kidney, mesenteric, inguinal fat depots recorded. Effects these phenotypes, along with depot weight carcass necropsy, mapped across genome using 1,402 autosomal single-nucleotide polymorphism (SNP) markers. Haplotypes reconstructed additive, dominance, imprinting genotype scores derived every 1 cM map. Analysis was performed mixed model scores, their interactions sex, diet, sex-by-diet as fixed effects family its interaction random effects. We discovered 95 trait-specific QTLs mapping 40 locations. Most had additive dominance occurring two-thirds loci. Nearly locus interacted sex and/or important ways demonstrating that gene are primarily context dependent, changing depending diet.