Inhibition of Transcription Factor Stat5 Induces Cell Death of Human Prostate Cancer Cells

摘要: Identifying regulators of prostate cancer cell survival may lead to new therapeutic strategies for cancer. We now report prevalent activation transcription factor Stat5 in human and provide novel evidence that blocking cells leads extensive death. Specifically, was activated 65% specimens examined based on nuclear location tyrosine phosphorylated Stat5. Adenoviral gene delivery a dominant-negative mutant (DNStat5), but not wild-type Stat5, induced death both the androgen-independent line CWR22Rv androgen-sensitive LnCap line. Endogenous active cells. In contrast, only low levels inactive proteins were detected PC-3 line, which correlated with resistance DNStat5-induced cells, inhibition by expression DNStat5 apoptotic as judged from morphological changes, DNA fragmentation, caspase-3 caspase-9-dependent mechanism. propose function represent approach