Synthesis and assembly of a functionally active recombinant platelet-derived growth factor AB heterodimer.
作者： A Ostman , L Rall , A Hammacher , M A Wormstead , D Coit
DOI: 10.1016/S0021-9258(18)37579-3
关键词:
摘要: A Chinese hamster ovary cell line that stably expresses transfected human platelet-derived growth factor (PDGF) and B chain precursors was established. All three dimeric combinations of PDGF chains were produced by this line; their biosynthesis, assembly, processing followed pulse-chase analyses. PDGF-AA, PDGF-AB, PDGF-BB processed to Mr values about 30,000 accumulated in these forms the medium. In addition, further a 24-kDa component, which remained cell-associated. The major secreted component purified shown have structural functional characteristics indistinguishable from PDGF-AB platelets.
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