They are not stealthy in the heart: embryonic stem cells trigger cell infiltration, humoral and T-lymphocyte-based host immune response

摘要: Objective: The in vivo immunogenicity of Embryonic Stem Cells is controversial. At present, there only vitro evidence MHC I expression by this cell population but vivid speculation about their immune-privileged state. immunology aspect ESC transplantation deserves thorough investigation. Methods: We injected mouse (expressing Green Fluorescent Protein, GFP) into injured myocardium syngeneic, allogeneic and SCID recipients. Furthermore, we monitored host response for up to 4 weeks post transfer. determined local (CD 3, CD 11c cells), donor cells, MHC-II within around the graft, humoral hosts using Flow Cytometry evaluated hosts’ cytokine stimulated spleenocytes means ELISPOT. Cell survival was estimated morphometry, calculating area GFP C graft over infarction at multiple sections harvested heart. Results: There significant cellular infiltration consisting T-lymphocytes (CD3 ) dendritic cells 11c). Infiltration detectable 1 week progressed through following transplantation. Ab moderate 2 frank weeks. ELISPOT demonstrated a Th1 pathway specific T-lymphocyte with strong IFN-g Il-2 production (figure A). maximal groups. Conclusions: An immune against transplanted future use will likely require systemic immunosuppression. Q 2005 Elsevier B.V. All rights reserved.