Detection of Enterotoxin Genes and Methicillin-Resistance in Staphylococcus aureus Isolated from Water Buffalo Milk and Dairy Products.

摘要: The aim of this study was to analyze the presence genes encoding staphylococcal enterotoxins and methicillin resistance in Staphylococcus aureus isolates obtained from water buffalo milk dairy products. A total 200 samples (100 raw milk, 50 clotted cream, cheese samples) collected different farms smallholders Samsun, Turkey. All were analyzed using standard procedure EN ISO 6888-1 confirmed for target 16S rRNA specific genus nuc gene S. species by PCR. identified 30 100 (30%), 9 cream (18%), 17 (34%) samples. 99 as aureus. Genotypic evaluated PCR mecA gene. Out isolates, nine (9%) found be resistant (mecA positive). Twelve out (12%) positive one or more enterotoxins. coding enterotoxin, sea, most frequent (five 41.6%), followed sec (two 16.6%), sed (1 8.3%) see isolate, 8.3%). While three (25%) contained both sed, none seb. In conclusion, se gene-positive methicillin-resistant products revealed that consumption these is a potential risk foodborne infection region. Practical application Enterotoxigenic (MRSA) an important public health problem. Especially traditional products, Staphylococcal may cause food poisoning due unpasteurized