Phagocytosis of Nickel Subsulfide Particles during the Early Stages of Neoplastic Transformation in Tissue Culture

摘要: Cellular uptake of crystalline Ni3S2 particles (a potent carcinogen) and amorphous NiS noncarcinogen) were studied using light electron microscopy. Cultured Syrian hamster embryo cells Chinese ovary actively phagocytized (less than or equal to 5 micrometer) but did not take up micrometer). For example, within 30 min after addition (high dose) cell cultures, 12.5% the contained nickel in cytoplasm. Within 6 hr, 75% had engulfed particles. Cultures exposed under similar conditions less 1.0% containing particulate hr. The was related particle concentration duration exposure. Concentration-dependent morphological transformation induced by cells, treatment with resulted no transformation. Pretreatment benzopyrene enhanced cellular also incidence Ni3S2-induced Our results suggest that carcinogenic activity is its uptake.