Modulation of thyroglobulin messenger RNA level by thyrotropin in cultured thyroid cells

摘要: To examine the influence of thyrotropin (TSH) on thyroglobulin (Tgb) mRNA content, latter was evaluated in cytoplasm hog thyroid cells cultured absence (control cells) or presence TSH. The Tgb levels were determined by, (i) kinetics hybridization to sheep cDNA, (ii) capacity coding for peptides immunologically related reticulocyte lysate. In 4 days TSH, content sequences decreased 30% its initial value and messenger activity 15%. Conversely, TSH maintained level presence, concentrations 50 micronU/ml 5 mU/ml gave identical results. At each period tested poly (A) same TSH-treated control cells. When added media after 8 culture without partially restored. These results suggest that exerts a positive gene expression through modulation