Distinctive patterns of Her-2/neu, c-myc, and cyclin D1 gene amplification by fluorescence in situ hybridization in primary human breast cancers.

摘要: Background: Human solid tumors undergo clonal evolution as they progress, but evidence for specific sequences of genetic changes that occur in individual and are recapitulated other is difficult to obtain. Methods: Patterns amplification Her-2/neu, c-myc, cyclin D1 were determined by fluorescence situ hybridization (FISH) relation the presence p53 dysfunction ploidy 60 primary human breast cancers. Results: We show there clusters genophenotypic abnormalities distinguish lobular cancers from nonlobular tumors; occurs prior divergence cancers; dysfunction, Her-2/neu amplification, c-myc characteristic features cancers, not frequencies all three oncogenes examined increase progressively with increasing aneuploidy, each gene exhibits a different profile tumor progression. Early appears be an especially prominent feature hypertetraploid/hypertetrasomic tumors. Conclusions: The data suggest containing multiple abnormalities, these often accumulate same cells within tumor. Furthermore, patterns accumulation Cytometry (Comm. Clin. Cytometry) 46:136–149, 2001. © 2001 Wiley-Liss, Inc.