Molecular Characterization and Functional Analysis of Three Autophagy Genes, BxATG5, BxATG9, and BxATG16, in Bursaphelenchus xylophilus.
作者： Hong-Bin Liu , Lin Rui , Ya-Qi Feng , Xiao-Qin Wu
DOI: 10.3390/IJMS20153769
关键词:
摘要: The pine wood nematode (PWN), Bursaphelenchus xylophilus, is the pathogen responsible for wilt disease (PWD), a devastating forest with pathogenic mechanism that remains unclear. Autophagy plays crucial role in physiological and pathological processes eukaryotes, but its regulatory significance PWN are unknown. Therefore, we cloned characterized three autophagy genes, BxATG5, BxATG9, BxATG16, PWN. BxATG9 BxATG16 were efficiently silenced through RNA interference, found positively regulated expression of BxATG5. Silencing severely inhibited feeding reproduction PWN, indicating essential these processes. We then examined patterns genes under stresses α-pinene H2O2, main defense substances trees, during development PWD using quantitative reverse transcription polymerase chain reaction. levels all significantly increased after nematodes stressed H2O2 inoculated into suggesting an important pathogenesis In this study, molecular characteristics functions elucidated.
mdpi.com
sci-hub.se 参考文章(46)
Han Nim Lee, Taijoon Chung, Overview of Autophagy in Plant Cells Journal of Life Sciences. ,vol. 24, pp. 209- 217 ,(2014) , 10.5352/JLS.2014.24.2.209
Minako Matsushita, Nobuo N. Suzuki, Keisuke Obara, Yuko Fujioka, Yoshinori Ohsumi, Fuyuhiko Inagaki, Structure of Atg5·Atg16, a Complex Essential for Autophagy Journal of Biological Chemistry. ,vol. 282, pp. 6763- 6772 ,(2007) , 10.1074/JBC.M609876200
P. E. Urwin, Catherine J. Lilley, Howard J. Atkinson, Ingestion of double-stranded RNA by preparasitic juvenile cyst nematodes leads to RNA interference. Molecular Plant-microbe Interactions. ,vol. 15, pp. 747- 752 ,(2002) , 10.1094/MPMI.2002.15.8.747
Makoto Asakura, Sachiko Ninomiya, Miki Sugimoto, Masahide Oku, Shun-ichi Yamashita, Tetsuro Okuno, Yasuyoshi Sakai, Yoshitaka Takano, Atg26-Mediated Pexophagy Is Required for Host Invasion by the Plant Pathogenic Fungus Colletotrichum orbiculare The Plant Cell. ,vol. 21, pp. 1291- 1304 ,(2009) , 10.1105/TPC.108.060996
Zhifen Yang, Daniel J Klionsky, Mammalian autophagy: core molecular machinery and signaling regulation Current Opinion in Cell Biology. ,vol. 22, pp. 124- 131 ,(2010) , 10.1016/J.CEB.2009.11.014
Tamara Vellosillo, Jorge Vicente, Satish Kulasekaran, Mats Hamberg, Carmen Castresana, Emerging Complexity in Reactive Oxygen Species Production and Signaling during the Response of Plants to Pathogens Plant Physiology. ,vol. 154, pp. 444- 448 ,(2010) , 10.1104/PP.110.161273
Noboru Mizushima, Masaaki Komatsu, Autophagy: Renovation of Cells and Tissues Cell. ,vol. 147, pp. 728- 741 ,(2011) , 10.1016/J.CELL.2011.10.026
Ghazaleh Rafatian, Fariba Khodagholi, Mahdi Moridi Farimani, Shahnaz Babaei Abraki, Mossa Gardaneh, Increase of autophagy and attenuation of apoptosis by Salvigenin promote survival of SH-SY5Y cells following treatment with H2O2 Molecular and Cellular Biochemistry. ,vol. 371, pp. 9- 22 ,(2012) , 10.1007/S11010-012-1416-6
Noboru Mizushima, Akiko Kuma, Yoshinori Kobayashi, Akitsugu Yamamoto, Masami Matsubae, Toshifumi Takao, Tohru Natsume, Yoshinori Ohsumi, Tamotsu Yoshimori, Mouse Apg16L, a novel WD-repeat protein, targets to the autophagic isolation membrane with the Apg12-Apg5 conjugate Journal of Cell Science. ,vol. 116, pp. 1679- 1688 ,(2003) , 10.1242/JCS.00381
Xiao-Lu Xu, Xiao-Qin Wu, Jian-Ren Ye, Lin Huang, Molecular characterization and functional analysis of three pathogenesis-related cytochrome P450 genes from Bursaphelenchus xylophilus (Tylenchida: Aphelenchoidoidea). International Journal of Molecular Sciences. ,vol. 16, pp. 5216- 5234 ,(2015) , 10.3390/IJMS16035216