Overexpression of transforming growth factor β-type II receptor reduces tumorigenicity and metastastic potential of K-ras-transformed thyroid cells
作者： Alessandra Turco , Anna Coppa , Simona Aloe , Gianluca Baccheschi , Stefania Morrone
DOI: 10.1002/(SICI)1097-0215(19990105)80:1<85::AID-IJC17>3.0.CO;2-P
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摘要: Expression of type II receptor transforming growth factor beta (TβRII) is necessary for this to inhibit the thyroid epithelial cells. In rat transformed cells, resistance (TGFβ) associated with a decreased expression TβRII mRNA and protein. Reduced has also been found in human differentiated undifferentiated carcinomas. To investigate role modulating tumorigenic potential k-ras-transformed we transfected these cells an vector carrying gene, regulated by inducible promoter. Isolated clones, overexpressing TβRII, showed reduction anchorage-dependent -independent cell growth, compared control When transplanted athymic nude mice, clones presented decrease tumorigenicity respect highly malignant parental Moreover, diminished ability studied was accompanied statistically significant spontaneous lung artificial metastases. Taken together, our data demonstrate that acts as potent tumor suppressor gene when overexpressed Int. J. Cancer 80:85–91, 1999. © 1999 Wiley-Liss, Inc.
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