Hsp70 chaperone rescues C6 rat glioblastoma cells from oxidative stress by sequestration of aggregating GAPDH.

摘要: The Hsp70 chaperone is known to elicit cytoprotective activity and this protection has a negative impact in anti-tumor therapy. In cancer cells subjected oxidative stress may bind damaged polypeptides proteins involved apoptosis signaling. Since one of the important targets glyceraldehyde-3-phospate dehydrogenase (GAPDH) we suggested that might its protective effect by binding GAPDH. Microscopy data show C6 rat glioma hydrogen peroxide treatment considerable proportion GAPDH molecules are denatured according dot ultrafiltration they form SDS-insoluble aggregates. Using two newly developed assays can oxidized an ATP-dependent manner. Pharmacological up- or down-regulation with aid U133 echinochrome triptolide, respectively, reduced increased number containing aggregates dying due peroxide. immunoprecipitation found able sequester aggregation-prone explain anti-oxidative power chaperone. results study led us conclude constantly exposed conditions stress, should be abolished specific inhibitors expression.