Formation and resolution of recombination intermediates by E. coli RecA and RuvC proteins.

作者: Hazel J. Dunderdale , Fiona E. Benson , Carol A. Parsons , Gary J. Sharpies , Robert G. Lloyd

DOI: 10.1038/354506A0

关键词:

摘要: … Using suitable DNA substrates, the combined action of RecA and RuvC leads to the formation of recombinant DNA molecules in vitro. Moreover, using small synthetic DNA junctions, we …

参考文章(43)
D. M. J. Lilley, The structure of the helical four-way junction in DNA, and its role in genetic recombination. Nucleic Acids and Molecular Biology. ,vol. 4, pp. 55- 77 ,(1990) , 10.1007/978-3-642-84150-7_4
S W Umlauf, M M Cox, R B Inman, Triple-helical DNA pairing intermediates formed by recA protein. Journal of Biological Chemistry. ,vol. 265, pp. 16898- 16912 ,(1990) , 10.1016/S0021-9258(17)44847-2
C M Radding, Helical interactions in homologous pairing and strand exchange driven by RecA protein. Journal of Biological Chemistry. ,vol. 266, pp. 5355- 5358 ,(1991) , 10.1016/S0021-9258(19)67599-X
H Shinagawa, K Makino, M Amemura, S Kimura, H Iwasaki, A Nakata, Structure and regulation of the Escherichia coli ruv operon involved in DNA repair and recombination. Journal of Bacteriology. ,vol. 170, pp. 4322- 4329 ,(1988) , 10.1128/JB.170.9.4322-4329.1988
B Jagadeeshwar Rao, Biru Jwang, Charles M Radding, None, RecA protein reinitiates strand exchange on isolated protein-free DNA intermediates: An ADP-resistant process Journal of Molecular Biology. ,vol. 213, pp. 789- 809 ,(1990) , 10.1016/S0022-2836(05)80264-5
C A Parsons, B Kemper, S C West, Interaction of a four-way junction in DNA with T4 endonuclease VII. Journal of Biological Chemistry. ,vol. 265, pp. 9285- 9289 ,(1990) , 10.1016/S0021-9258(19)38845-3
H Iwasaki, T Shiba, K Makino, A Nakata, H Shinagawa, Overproduction, purification, and ATPase activity of the Escherichia coli RuvB protein involved in DNA repair. Journal of Bacteriology. ,vol. 171, pp. 5276- 5280 ,(1989) , 10.1128/JB.171.10.5276-5280.1989