Unique Epitopes of Glutamic Acid Decarboxylase Autoantibodies in Slowly Progressive Type 1 Diabetes
作者： Tetsuro Kobayashi , Shoichiro Tanaka , Minoru Okubo , Koji Nakanishi , Toshio Murase
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摘要: Disease-specific epitope profiles of glutamic acid decarboxylase (GAD)65 autoantibodies (GAD65Ab) were studied in slowly progressive type 1 (insulin-dependent) diabetes mellitus (SPIDDM) and acute onset (AIDDM) using seven kinds GAD65/67 chimeric molecules. Sera obtained from Japanese SPIDDM (n = 17) AIDDM 46) patients followed prospectively analyzed by immunoprecipitation, ELISA, Western blotting. GAD65Ab all samples reacted specifically with an N-terminal linear located on the membrane anchoring domain between amino acids 17–51 C-terminal conformational 443–585 GAD65. The binding 83 residues inversely correlated period which insulin was not required. did react 1–83, irrespective titer GAD65Ab. A novel residing acids...
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