High-throughput lung cancer cell line screening for genotype-correlated sensitivity to an EGFR kinase inhibitor.

摘要: Human cancer cell lines that can be propagated and manipulated in culture have proven to excellent models for studying many aspects of gene function cancer. In addition, they provide a powerful system assessing the molecular determinants sensitivity anticancer drugs. They also been used recent studies identify genomic alterations expression patterns important insights into genetic features distinguish properties tumor cells associated with similar histologies. We established large repository human (>1000) corresponding wide variety types, we developed methodology profiling collection putative compounds. The rationale examining on this relatively scale reflects accumulating evidence indicating there is substantial heterogeneity among cells-even those derived from tumors Thus, develop an accurate picture tumorigenesis response therapy, it essential study nature such sample set. Here, describe methodologies conduct screens "proof-of-concept" screen using EGFR kinase inhibitor, erlotinib (Tarceva), panel lung demonstrate correlation between mutations drug sensitivity.