The Tomato Kinome and the Tomato Kinase Library ORFeome: Novel Resources for the Study of Kinases and Signal Transduction in Tomato and Solanaceae Species

摘要: Protein kinase-driven phosphorylation constitutes the core of cellular signaling. Kinase components signal transduction pathways are often targeted for inactivation by pathogens. The study kinases and immune in model crop tomato (Solanum lycopersicum) would benefit from availability community-wide resources large scale systems-level experimentation. Here, we defined kinome performed a comprehensive comparative analysis 15 other plant species. We constructed kinase library (TOKN 1.0) over 300 full-length open reading frames (ORF) cloned into recombination-based vector. developed high-throughput pipeline to isolate transform protoplasts. A subset TOKN 1.0 were expressed planta, purified, used generate functional protein microarray. All created utilized test known novel associations between Pseudomonas syringae DC3000 effectors large-scale format. Bsk7 was identified as component response candidate effector target. These will enable investigations signaling host-pathogen interactions Solanaceae spp.