Ru(II)/diphenylphosphine/pyridine-6-thiolate complexes induce S-180 cell apoptosis through intrinsic mitochondrial pathway involving inhibition of Bcl-2 and p53/Bax activation.

作者: Wanessa Carvalho Pires , Benedicto Augusto Vieira Lima , Flávia de Castro Pereira , Aliny Pereira Lima , Francyelli Mello-Andrade

DOI: 10.1007/S11010-017-3129-3

关键词:

摘要: The aim of this work was the synthesis, characterization, and cytotoxicity evaluation three new Ru(II) complexes with a general formula [Ru(Spy)(bipy)(P-P)]PF6 [Spy = pyridine-6-thiolate; bipy 2,2′-bipyridine; P-P 1,2-bis(diphenylphosphine)ethane (1); 1,3-bis(diphenylphosphine) propane (2); 1,1′-bis(diphenylphosphino)ferrocene] (4). Complex (3) 1,4-bis(diphenylphosphine)butane ligand, already known from literature, also synthesized, to be better studied here. cytotoxicities toward two kinds cancerous cells (K562 S-180 cells) were evaluated compared normal (L-929 PBMC) by MTT assay. complex [Ru(Spy)(bipy)(dppb)]PF6 selected study both cellular molecular mechanisms underlying its promising anticancer action in cells. results obtained indicated that induces cell cycle arrest G0/G1 phase associated decrease number S phase. After 24 48 h exposure (3), viability decreased when negative control. does not appear involved DNA damage, but induced changes mitochondrial membrane potential Furthermore, there an increase gene expression Bax, Caspase 9, Tp53. According our results, apoptosis through p53/Bax-dependent intrinsic pathway suppresses active antiapoptotic Bcl-2 protein.

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