Amplification of ribosomal DNA in Acheta. I. The number of chromomeres involved in the amplification process.

摘要: Photometric measurements of DNA in Acheta domesticus were made on single nuclei and each pachytene chromosome, chromomere by chromomere. The extinction values processed a computer. background is negligible there good agreement between the pattern amount found. The 2C value brain 4.0 pg. following obtained for oocyte at early pachytene. (1) All bodies or major chromomeres 4.18 (2) chromosomes only, 9.11 (3) amplified chromosome 6, 2.00 (4) 11, 1.48 pg. The 11 oocytes contain 0.4, 0.3, 0.2, 0.1, 0.1 X 109 nucleotide pairs respectively (haploid value). amplicon (the site amplification) 6 contains 0.02 pg whereas that has 0.01 value); this corresponds to 108 0.01X respectively. In case 1.9-2.5 pairs, which circa 100 times its (haploid). An average (haploid) pairs. The location specific well-defined genes such as cistrons 28S 18S ribosomal RNA, coupled with knowledge number these helps elucidate organization function genetic units eukaryotes.