Discoidin domain receptor 1: isoform expression and potential functions in cirrhotic human liver.

摘要: Discoidin domain receptor 1 (DDR1) is a tyrosine kinase that binds and activated by collagens. Transcriptional profiling of cirrhosis in human liver using DNA array quantitative PCR detected elevated mRNA expression DDR1 compared with nondiseased liver. The present study characterized cirrhotic examined the cellular effects expression. all five isoforms was liver, whereas DDR1a demonstrated differential hepatitis C virus primary biliary In addition, immunoblot analysis shed fragments more readily than Inasmuch as subject to protease-mediated cleavage after prolonged interaction collagen, this may indicate intense activation protein situ hybridization immunofluorescence localized epithelial cells including hepatocytes at portal-parenchymal interface luminal aspect epithelium. Overexpression altered hepatocyte behavior increased adhesion less migration on extracelular matrix substrates. regulated extracellular metalloproteinases 2. These data elucidate function pertinent importance cell–collagen interactions chronic injury.