Evaluation of dengue virus infection in immune cells of human skin

摘要: Dengue virus (DENV) is a reemerging infectious agent causing an estimated 50-100 million cases annually and endemic in more than 100 countries. Recently there has been increase the geographic spread, overall number, severity of disease cases, making DENV increasing threat to public health. The most prevalent arboviruses, introduced into human hosts during vector mosquito's blood meal; here, deposited epidermal dermal layers skin. Therefore, elucidating cutaneous immune response critical our understanding mechanisms involved protection pathogenesis development effective treatments vaccines. In addition resident T- B-cells, skin system includes three major populations innate cells, dendritic cells (dDCs), macrophages (dMOs), Langerhans' (LCs). These are potential targets infection. receptor profile dDCs dMOs indicates that they susceptible infection, however evidence this lacking. LCs infected following ex vivo inoculation explants, yet their role remains unclear. Upon exposure pathogen, mature migrate draining lymph node via lymphatics; these functions induction adaptive responses pathogen clearance. It unclear how infection affects maturation, migration survival cells. Herein, we use combination vitro studies healthy explants explore We investigated degree which become with measured upregulation activation markers, out skin, life span discovered DCs, as well dMOs, upon scarification virus. vitro, confirmed partially inhibits maturation DCs potentially MOs. Additionally, induces DC apoptosis caspase-dependent manner. results help clarify immunopathogenesis DENV.