Interferon-gamma, but not interferon-alpha, induces SOCS 3 expression in human melanoma cell lines.
作者： Ales Kovarik , Miloslava Fojtova , Vladimir Boudny , Lenka Adamkova , Ludmila Lauerova
DOI: 10.1097/00008390-200512000-00001
关键词:
摘要: The signal transducers and transcription activators (STATs) their endogenous inhibitors of the suppressors cytokine signalling (SOCS) family are major proteins harmonizing transmission external signals from surface membrane to target genes in nucleus. To correlate induction SOCS 3 by interferons (IFNs) on messenger RNA protein levels with STAT 1 phosphorylation human malignant melanoma cell lines, we used a unique collection 18 established lines six non-malignant normal cells (two melanocytes, two skin keratinocytes fibroblasts). IFN-gamma induced 83% whereas IFN-alpha stimulated expression only 11% cases. Similarly, melanocytes showed strong and, lesser extent, IFN-alpha. In most cases, was paralleled at tyrosine residues (Y701). several however, not despite few occurred without detectable phosphorylation, indicating that might be an exclusive inducer 3. displayed activation high after (but IFN-alpha) treatment. conclusion, contrast IFN-alpha, appeared induce apparently level exhibited higher cytotoxic effects regardless origin.
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