Can experimental models of rodent implantation glioma be improved? A study of pure and mixed glioma cell line tumours.

摘要: To evaluate the hypothesis that co-implantation of different rodent glioma cell lines might result in experimental brain tumours more closely resemble human gliomas neuropathology and immunocytochemical features implantation derived from single (C6, A15A5, F98), two admixed 50:50 prior to (C6 + F98 C6 A15A5) three equally A15A5 F98) was studied adult Wistar rat. Tumours grew consistently following lines, however tumour growth triple mix considerably impaired. The showed regional heterogeneity with areas characteristic both primary lines. Foci lymphocytic infiltrates, tumoural necrosis, often pseudopallisading, peritumoural edema were consistent all tumours. Limited parenchymal extensive perivascular invasion seen predominantly containing line. There no significant differences GFAP, vimentin HSP70 staining between mixed tumours, although pure were, unlike gliomas, S-100 negative. Using PCNA expression as a measure proliferation except mix, which had index 7–10%, focal indices viable 40–80%. positive perilesional regions for macrophage markers ED-1 ED-2. None stained vitro either ED1 ED2 but constitutively OX-6, proposed marker antigen presenting cells. response suggest vigorous largely ineffective immunological been mounted against failure grow is unexplained. This work has shown feasibility producing ‘mixed’ line by combining at time innoculation. However, relative produce (i) have properties not inherent parent (ii) there are limits this approach. Admixture therefore does make models natural also some particular disadvantages. approach recommended use study biology evaluating effectiveness novel therapies.