Bioactivation of dermal scaffolds with a non-viral copolymer-protected gene vector.

摘要: The use of scaffolds in skin tissue engineering is accompanied with low regeneration rates and high risk infection. In this study, we activated an FDA-approved collagen scaffold for dermal by incorporation copolymer-protected gene vectors (COPROGs) to induce a temporary release VEGF. vitro results show that the presence COPROGs did not affect distribution, attachment, proliferation viability cells scaffold. A transient VEGF was observed up 3 weeks. Moreover amount also found associated full defect model nude mice, levels were significantly increased compared controls 14 d after implantation, but from wound edge. Results showed non-adherent cells, especially erythrocytes, von Willebrandt factor (vWF) yellow red appearance relation controls. This suggests leaky vessels. work bioactivation presents new technology allows local therapeutic proteins thus enhancing regenerative potential vivo.