Hookworm products ameliorate dextran sodium sulfate‐induced colitis in BALB/c mice

摘要: Background: Several lines of evidence have shown that helminthiasis can significantly reduce disease severity in animal models intestinal inflammation, airway inflammation/hyperreactivity, diabetes, and multiple sclerosis. Identification characterization helminth-derived immunomodulatory molecules contribute to anticolitis effects could lead new therapeutic approaches inflammatory bowel diseases (IBDs) without the need for helminth infection. We evaluated potential adult human hookworm, Ancylostoma ceylanicum, crude (Aw) excreted/secreted (ES) products on dextran sulfate sodium (DSS)-induced colitis BALB/c mice. Methods: Colitis was induced by 5% DSS oral administration 7 days. Clinical monitored daily during concomitant intraperitoneal treatment with products. Additionally, several pathways immunological modulation A. ceylanicum (MPO, EPO, Th1, Th2, Th17 cytokine responses) inflamed microenvironment were assessed. Finally, histopathological profile colon characterized. Results: Hookworm are able modulate potent proinflammatory response DSS, mainly through downregulation Th1 cytokines. These proteins also clinical colonic microscopic inflammation scores as well EPO MPO activity. Conclusions: Ancylostoma Aw ES mediators an important experimental mice, which may provide a more socially acceptable form therapy patients IBDs opposed using living worms. Our results support urgency further isolation recombinant expression active hookworm responsible beneficial colitis. (Inflamm Bowel Dis 201117:2275–2286)