Prognostic significance of telomerase-associated parameters in glioblastoma: effect of patient age

摘要: Background. Glioblastoma multiforme (GBM) is a heterogeneous, highly aggressive primary brain tumor with strongly variable patient survival. Because reliable prognostic biomarkers are lacking, we investigated the relation between telomerase-associated parameters and disease course. Methods. Telomerase-associated were determined in 100 GBM tissues associated clinical characteristics overall Expressions of telomere length, telomerase activity (TA), human reverse transcriptase (hTERT) analyzed by quantitative PCR, telomeric repeat amplification protocol assay, transcriptase‐PCR, respectively. Mutation status isocitrate dehydrogenase (IDH)1 was direct sequencing, O 6 -methylguanine DNA methyltransferase (MGMT) promoter methylation methylation-specific PCR. Results. Of tissues, 61 positive for both hTERT mRNA TA, significant correlation (linear regression, P , .0001). Telomere length determination revealed difference hTERT/TA-positive -negative subgroups, markedly longer telomeres