ATP is released from rabbit urinary bladder epithelial cells by hydrostatic pressure changes–possible sensory mechanism?

摘要: 1The responses of rabbit urinary bladder to hydrostatic pressure changes and electrical stimulation have been investigated using both the Ussing chamber a superfusion apparatus. These experiments enabled us monitor in ionic transport across tissue cellular ATP release from it. 2The maintains an potential difference its wall as result largely active sodium (mucosal) serosal surface. 3Small differences produced by removal bathing fluid one side caused reproducible short-circuit current. The magnitude these increases volume removed increases. 3Amiloride on mucosal (urinary), but not serosal, surface membrane reduces transepithelial current with IC50 300 nm. Amiloride size of, does abolish, alterations pressure. 4Field strips produces ATP. Such was demonstrated occur urothelial cells is apparently non-vesicular it absence calcium abolished tetrodotoxin. 5It proposed that released urothelium sensory mediator for degree distension other modalities.