Isolation and characterization of activin receptor from mouse embryonal carcinoma cells. Identification of its serine/threonine/tyrosine protein kinase activity.
作者： T Nakamura , K Sugino , N Kurosawa , M Sawai , K Takio
DOI: 10.1016/S0021-9258(19)37049-8
关键词:
摘要: The activin receptor protein was isolated from the mouse embryonal carcinoma (EC) cell line P19 by three cycles of affinity chromatography on an A-immobilized column. purified had a specific and high for activins A, AB, B (Kd = 345 pM), but not transforming growth factor beta. identified sodium dodecyl sulfate-polyacrylamide gel electrophoresis ligand blotting analysis as single 70 kDa. amino acid sequence first 18 NH2-terminal residues revealed that is member family. phosphorylated itself exogenous substrate proteins serine, threonine, tyrosine residues, indicating transmembrane serine/threonine/tyrosine kinase. These results suggest signal transduction employs novel pathway via new class cellular in EC cells.
sci-hub.st 参考文章(38)
M Hino, A Tojo, K Miyazono, Y Miura, S Chiba, Y Eto, H Shibai, F Takaku, Characterization of cellular receptors for erythroid differentiation factor on murine erythroleukemia cells. Journal of Biological Chemistry. ,vol. 264, pp. 10309- 10314 ,(1989) , 10.1016/S0021-9258(18)81801-4
H Sugino, T Nakamura, Y Hasegawa, K Miyamoto, M Igarashi, Y Eto, H Shibai, K Titani, Identification of a specific receptor for erythroid differentiation factor on follicular granulosa cell. Journal of Biological Chemistry. ,vol. 263, pp. 15249- 15252 ,(1988) , 10.1016/S0021-9258(19)37578-7
T Nakamura, M Asashima, Y Eto, K Takio, H Uchiyama, N Moriya, T Ariizumi, T Yashiro, K Sugino, K Titani, Isolation and characterization of native activin B. Journal of Biological Chemistry. ,vol. 267, pp. 16385- 16389 ,(1992) , 10.1016/S0021-9258(18)42014-5
T. Nakamura, K. Sugino, K. Titani, H. Sugino, Follistatin, an activin-binding protein, associates with heparan sulfate chains of proteoglycans on follicular granulosa cells. Journal of Biological Chemistry. ,vol. 266, pp. 19432- 19437 ,(1991) , 10.1016/S0021-9258(18)55015-8
M Hashimoto, T Nakamura, S Inoue, T Kondo, R Yamada, Y Eto, H Sugino, M Muramatsu, Follistatin is a developmentally regulated cytokine in neural differentiation. Journal of Biological Chemistry. ,vol. 267, pp. 7203- 7206 ,(1992) , 10.1016/S0021-9258(18)42503-3
Jonathan A. Cooper, Bartholomew M. Sefton, Tony Hunter, [42] Detection and quantification of phosphotyrosine in proteins Methods in Enzymology. ,vol. 99, pp. 387- 402 ,(1983) , 10.1016/0076-6879(83)99075-4
Carolyn A. Campen, Wylie Vale, Characterization of activin A binding sites on the human leukemia cell line K562 Biochemical and Biophysical Research Communications. ,vol. 157, pp. 844- 849 ,(1988) , 10.1016/S0006-291X(88)80326-7
H. E. Broxmeyer, L. Lu, S. Cooper, R. H. Schwall, A. J. Mason, K. Nikolics, Selective and indirect modulation of human multipotential and erythroid hematopoietic progenitor cell proliferation by recombinant human activin and inhibin Proceedings of the National Academy of Sciences of the United States of America. ,vol. 85, pp. 9052- 9056 ,(1988) , 10.1073/PNAS.85.23.9052
A. J. M. van den Eijnden-Van Raaij, E. J. J. van Zoelent, K. van Nimmen, C. H. Koster, G. T. Snoek, A. J. Durston, D. Huylebroeck, Activin-like factor from a Xenopus laevis cell line responsible for mesoderm induction. Nature. ,vol. 345, pp. 732- 734 ,(1990) , 10.1038/345732A0
Jeremy B. A. Green, J. C. Smith, Graded changes in dose of a Xenopus activin A homologue elicit stepwise transitions in embryonic cell fate Nature. ,vol. 347, pp. 391- 394 ,(1990) , 10.1038/347391A0