Ionizing Radiation and Glioblastoma Exosomes: Implications in Tumor Biology and Cell Migration

摘要: Exosomes are nanometer-sized lipid vesicles released ubiquitously by cells, which have been shown to a normal physiological role, as well influence the tumor microenvironment and aid metastasis. Recent studies highlight ability of exosomes convey tumor-suppressive oncogenic mRNAs, microRNAs, proteins receiving cell, subsequently activating downstream signaling pathways influencing cellular phenotype. Here, we show that radiation increases abundance glioblastoma cells astrocytes. derived from irradiated enhanced migration recipient their molecular profiling revealed an molecules related important for cell migration. In particular, connective tissue growth factor (CTGF) mRNA insulin-like binding protein 2 (IGFBP2) levels were elevated, coculture nonirradiated with isolated increased CTGF expression in cells. Additionally, these activation neurotrophic tyrosine kinase receptor type 1 (TrkA), focal adhesion kinase, Paxillin, proto-oncogene tyrosine-protein Src (Src) involved Collectively, our data suggest influences exosome abundance, specifically alters composition, on uptake, promotes migratory