Secretome profiling identifies neuron-derived neurotrophic factor as a tumor-suppressive factor in lung cancer

摘要: Clinical and preclinical studies show tissue-specific differences in tumorigenesis. Tissue specificity is controlled by differential gene expression. We prioritized genes that encode secreted proteins according to their preferential expression normal lungs identify candidates associated with lung cancer. Indeed, most of the lung-enriched identified our analysis have known or suspected roles focused on encoding neuron-derived neurotrophic factor (NDNF), which had not yet been determined NDNF was preferentially expressed adult its decreased human adenocarcinoma a mouse model this Higher better clinical outcome patients adenocarcinoma. Purified inhibited proliferation cancer cells, whereas silencing promoted tumor cell growth culture xenograft models. downregulated through DNA hypermethylation near CpG island shores Furthermore, cancer-related sites corresponded methylation occurred tissues low Thus, analyzing secretome, we tumor-suppressive factor, NDNF, patient outcomes