Isolation and composition of human thymocyte plasma membrane

作者: David Allan , M.J. Crumpton

DOI: 10.1016/0005-2736(72)90276-3

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摘要: Abstract Plasma membrane was isolated from human thymus cells using an identical procedure with that described previously for the plasma of pig lymphocytes. Its chemical and enzymic compositions, apart a lower more variable 5′-nucleotidase activity, were similar to those lymphocyte membrane. Polyacrylamide gel electrophoresis thymocyte in sodium dodecyl sulphate gave large number protein bands about seven which stained also carbohydrate. A comparison carbohydrate patterns membranes revealed differences glycoprotein composition.

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