Acteoside: a lipase inhibitor from the Chinese tea Ligustrum purpurascens kudingcha.
作者： Xuli Wu , Weiyi He , Haiping Zhang , Yao Li , Zhigang Liu
DOI: 10.1016/J.FOODCHEM.2013.07.071
关键词:
摘要: Acteoside is the most abundant and major active component of Ligustrum purpurascens (kudingcha tea). Here, we explored anti-obesity properties acteoside by investigating its effect on lipase activity. Characterization fluorescence spectroscopy, isothermal titration calorimetry circular dichroism revealed that might act as a non-competitive inhibitor. bound to at Ka=1.88×10(4)lmol(-1). Thermodynamic features suggested binding interaction was mainly hydrophobic complex stabilized hydrogen bonding, with 1:1 lipase. Furthermore, docking results supported experimental findings bonding Lys271, Leu272 Thr68 This non-covalent between alters molecular conformation lipase, which decreases enzyme catalytic
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