LIM mineralization protein-1 suppresses TNF-α induced intervertebral disc degeneration by maintaining nucleus pulposus extracellular matrix production and inhibiting matrix metalloproteinases expression
作者： Hui Liu , Hehai Pan , Hao Yang , Jianru Wang , Kuibo Zhang
DOI: 10.1002/JOR.22732
关键词:
摘要: Imbalanced metabolism of Nucleus pulposus (NP) extracellular matrix (ECM) is closely correlated to Intervertebral Disc Degenerative Disease. LIM mineralization protein-1 (LMP-1) has been proven induce sulfated glycosaminoglycan (sGAG) production in NP and have an anti-inflammatory effect pre-osteoclast. However, whether it any on the ECM degradation under inflammatory stimulation not studied. In current study, a TNF-α induced cell model was established vitro. Lentivirus encoding LMP-1 (LV-LMP-1) short heparin (LV-shLMP-1) were constructed overexpress knockdown expression cells. mRNA level regulated dose-dependent manner after transfection. LV-LMP-1 increased whereas LV-shLMP-1 decreased collagen II, aggrecan, versican expression, sGAG production. abolished while aggravated mediated down-regulation above genes via ERK1/2 activation. Moreover, abrogated MMP-3 MMP-13 inhibiting p65 translocation promoter activity. These results indicated that had maintenance stimulation. This up-regulation at least partially through activation, MMPs NF-κB inhibition. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:???–???, 2015.
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