Identification and biosynthesis of the bacteriophage T4 mot regulatory protein.

摘要: The T4 mot gene regulates middle mode RNA synthesis in phage-infected cells. product has been identified two ways. (i) Infections with amber and temperature-sensitive mutants both lead to the disappearance of a number protein bands on SDS-polyacrylamide gels. These are proteins whose depends function. disappears from such gels after infection mutant, but not missense mutant. (ii) This same is only one have charge alteration when wild-type phage mutant infections compared by two-dimensional gel electrophoresis. Mot basic mol. wt. 24 000. It migrates between positions gp 1 IPIII 15% begins immediately continues until 4 min at 30 degrees C, which time it strongly inhibited. inhibition neither DNA nor ADP ribosylation alpha subunits Escherichia coli polymerase. does regulate its own biosynthesis. stable throughout course infection.