The T4 mot protein functions as part of a pre-replicative DNA-protein complex.

摘要: Middle-mode RNA synthesis in T4-infected cells takes place before replication of phage DNA commences. What distinguishes it from early-mode is that initiation middle depends on T4-coded proteins, particular the mot gene product. protein localized a DNA-protein complex which forms during first few minutes infection. All cell's bound this complex, and continues to be long after has stopped. When we infect Escherichia coli with T4 carrying temperature-sensitive mutation gene, find correlation between physiology mutant amount complex. Although there some host polymerase does not seem bind enzyme. Two other molecular weights 17,600 15,000, are also found pre-replicative One these, p17,600, coded for by 750-base pair region located genes 39 56; p17,600 appears recently described motB The protein, p15,000, an polymerase-binding protein; characterized its strong binding