Possible mechanism of perfluorooctane sulfonate and perfluorooctanoate on the release of calcium ion from calcium stores in primary cultures of rat hippocampal neurons.

摘要: Perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) are anthropogenic compounds manufactured since the 1950s distributed worldwide. Now, pollutants being challenged by entering into brain toxic effect on central nervous system due to calcium disorder, mainly through channels cell membrane. However, little is known about role of store in PFOS- PFOA-evoked abnormal increase. In present study, PFOA PFOS were measured primary cultures rat hippocampal neurons LC/MS/MS analysis. Flow cytometry was used examine altered patterns labeled with fluo-3/AM disclose mechanism which induced increase cultured neurons. The results indicate that both can accumulate elevate concentrations via release intracellular stores. Furthermore, inositol 1,4,5-trisphosphate receptors (IP(3)Rs) ryanodine (RyRs) found take part or inducing from IP(3)Rs seem serve a predominant PFOS-induced release. Calcium stores may partially account for perturbation homeostasis caused PFOA.