Phenotype and Genotype of Advanced Premalignant Head and Neck Lesions After Chemopreventive Therapy

摘要: Background: The goal of chemoprevention is to reduce the risk cancer development by reversing or blocking tumorigenic process through use pharmacologic natural agents. To determine potential role genetic alterations in assessing and evaluating efficacy chemopreventive agents, we studied 22 patients with advanced premalignant lesions head neck who were part a prospective prevention trial that investigating regimen 13-cis-retinoic acid, interferon alfa, a-tocopherol administered for 12 months until disease progression. Methods: We used polymerase chain reaction analysis microsatellite DNA sequences cells from precancerous frequencies alterations—namely, loss heterozygosity (LOH) instability—at chromosomal loci are commonly deleted cancer. Results: Prior treatment, 17 (81%) 21, eight (44%) 18, (42%) 19 informative (i.e., heterozygous) at chromosomes 9p21, 3p14, 17p13, respectively, exhibited LOH least one their lesion biopsy specimens. Among nine chromosome 9p21 pretreatment specimens had completed 5 therapy, persisted eight—including three four complete histologic responses no evidence dysplasia specimens). Implication: Our data suggest clinical assessments response agents may be insufficient critical could as independent biomarkers augment ability evaluate such [J Natl Cancer Inst 1998;90:1545‐51]