Targeted resequencing of coding DNA sequences for SNP discovery in nonmodel species

摘要: Targeted capture coupled with high‐throughput sequencing can be used to gain information about nuclear sequence variation at hundreds thousands of loci. Divergent reference makes use molecular data one species enrich target loci in other (related) species. This is particularly valuable for nonmodel organisms, which often no a priori knowledge exists regarding these Here, we have targeted obtain 809 coding DNA sequences (CDS) organism, the Eurasian lynx Lynx lynx, using baits designed help published genome related model organism (the domestic cat Felis catus). Using this approach, were able survey intraspecific L. across species’ European range. A large set biallelic candidate SNPs was then evaluated SNP genotyping platform (Fluidigm), reduced final 96 SNP‐panel based on assay performance and reliability ; validation carried out 100 additional samples not included discovery phase. The developed from CDS performed very successfully identification individuals population genetic structure inference (including assignment their source population). In keeping recent studies, our results show that genic monitoring wildlife