KRAS mutations are associated with solid growth pattern and tumor-infiltrating leukocytes in lung adenocarcinoma

摘要: KRAS mutations define a clinically distinct subgroup of lung adenocarcinoma patients, characterized by smoking history, resistance to EGFR-targeted therapies, and adverse prognosis. Whether KRAS-mutated adenocarcinomas also have histopathological features is not well established. We tested 180 resected for EGFR high-sensitivity mass spectrometry-based genotyping (Sequenom) PCR-based sizing assays. All tumors were assessed the proportion standard histological patterns (lepidic, acinar, papillary, micropapillary, solid, mucinous), several other clinical parameters, TTF-1 expression immunohistochemistry. Among carcinomas, 63 (35%) had (KRAS+), 35 (19%) (EGFR+), 82 (46%) neither mutation (KRAS-/EGFR-). Solid growth pattern was significantly over-represented in KRAS+ carcinomas: mean±s.d. amount solid carcinomas 27±34% compared with 3±10% EGFR+ (P<0.001) 15±27% KRAS-/EGFR- (P=0.033) tumors. Furthermore, at least focal (≥20%) component more common (28/63; 44%) (2/35; 6%; P<0.001) (21/82; 26%; P=0.022) carcinomas. mucinous associated presence tumor-infiltrating leukocytes heavier history. non-mucinous non-solid patterns, particularly lepidic lack necrosis, cytological atypia, hobnail cytology, expression, never/light In conclusion, extended molecular clinicopathological analysis reveals novel association histology inflammatory cells expands on previously recognized morphological associations mutations. recently shown be strong predictor aggressive behavior adenocarcinomas, which may underlie unfavorable prognosis these