Expression of H5 hemagglutinin vaccine antigen in common duckweed (Lemna minor) protects against H5N1 high pathogenicity avian influenza virus challenge in immunized chickens.
作者： Kateri Bertran , Colleen Thomas , Xuan Guo , Michel Bublot , Nikki Pritchard
DOI: 10.1016/J.VACCINE.2015.05.076
关键词:
摘要: A synthetic hemagglutinin (HA) gene from the highly pathogenic avian influenza (HPAI) virus A/chicken/Indonesia/7/2003 (H5N1) (Indo/03) was expressed in aquatic plant Lemna minor (rLemna-HA). In Experiment 1, efficacy of rLemna-HA tested on birds immunized with 0.2μg or 2.3 μg HA and challenged 10(6) mean chicken embryo infectious doses (EID50) homologous strain. Both dosages conferred clinical protection dramatically reduced viral shedding. Almost all either dosage elicited antibody titers against Indo/03 antigen, suggesting an association between levels anti-Indo/03 antibodies protection. 2, 0.9 2.2 EID50 heterologous H5N1 strains A/chicken/Vietnam/NCVD-421/2010 (VN/10) A/chicken/West Java/PWT-WIJ/2006 (PWT/06). Birds VN/10 exhibited 100% survival regardless immunization dosage, while PWT/06 had 50% 30% mortality at HA, respectively. For each challenge virus, shedding vaccinated were significantly lower than those 0.9μg birds, both groups turn sham birds. Even if vaccine antigen Indo/03, only developed humoral immunity antigen. None (rLemna-HA HA) 40% pre-challenge titers, conclusion, Lemna-expressed demonstrated complete protective suboptimal challenge, latter being similar to results inactivated whole vaccines. Transgenic duckweed-derived could be a good alternative for producing high quality injectable HPAI viruses.
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