Relationship of amplified dihydrofolate reductase genes to double minute chromosomes in unstably resistant mouse fibroblast cell lines.
作者： P C Brown , S M Beverley , R T Schimke
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摘要: Murine 3T6 selected in increasing concentrations of methotrexate were unstable with respect to dihydrofolate reductase overproduction and resistance when they are cultured the absence methotrexate. An analysis karyotypes these resistant cells revealed presence numerous double minute chromosomes. We observed essentially identical kinetics loss gene sequences total deoxyribonucleic acid from fractions enriched chromosomes numbers per cell during reversion sensitivity, this suggested that unstably amplified localized on This conclusion ws also supported by an populations sorted according enzyme contents, which relative amplification chromosome content related proportionally.
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