Quantification of egg proteome changes during fertilization in sterlet Acipenser ruthenus.

摘要: Eggs of sterlet are discharged outside into ambient aquatic environment where egg activation and fertilization occur. Effects different media including freshwater clay suspension on protein abundances were quantified in Acipenser ruthenus. In-gel digestion high resolution mass spectrometry used for label-free quantification the eggs five females. No significant (p > 0.05) difference was found between activated with media. However, results showed < 0.05, fold change ≥2) reduction nine proteins six glycoproteins, enolase heat shock groups compared to freshly ovulated as control. The fact that abundance proteasome subunit alpha significantly reduced only which by suggests medium can somehow intervene regulation during fertilization. In conclusion, external sturgeon is accompanied huge release may participate construction a transient microenvironment around attraction protection spermatozoa ensure ensuing Data available via ProteomeXchange identifier PXD006232.