Hepatocyte growth factor (HGF) receptor expression is inducible and is part of the delayed-early response to HGF.

摘要: The c-MET proto-oncogene encodes the tyrosine kinase receptor for hepatocyte growth factor (HGF), also known as scatter factor, a potent mitogen and motogen epithelial cells. level of HGF expressed by cells varies in different conditions, being lower arrested confluent monolayers higher growing sparse amount mRNA increases from 3- to 5-fold after stimulation serum up 10-fold protein C 12-O-tetradecanoylphorbol-13-acetate (TPA). An increased was observed cell with nanomolar concentration itself. effect transient, dose, time-dependent. Transcription reporter gene under control cloned 297 base pair promoter stimulated serum, TPA, or HGF. accumulation specific is followed appearance precursor protein, which further processed mature form. After stimulation, expression follows c-FOS c-JUN induction peak approximately 4 h. Pretreatment synthesis inhibitor puromycin strongly reduced response HGF, while cycloheximide alone mRNA. These data show that behaves delayed early-response suggest autoamplified inducing receptor.