Thiolation of uridine carbon-2 restricts the motional dynamics of the transfer RNA wobble position nucleoside

摘要: Thiolation of transfer RNA wobble position uridines produces a preferred conformation the nucleoside in solution at ambient temperature that is biological significance to codon recognition [Sierzputowska-Gracz, H.; Sochacka, E.; Malkiewicz, A.; Kuo, K.; Gehrke, C.; Agris, P. F. J. Am. Chem. Soc. 1987, 109, 7171-7177]. We investigated and compared, by proton nuclear magnetic resonance (NMR) spectroscopy, thermodynamic stability conformations 2-thiouridine five biologically important 5-position derivatives six analogous