The importance of cell density in the interpretation of growth factor effects on collagenase IV activity release and extracellular matrix production from C6 astrocytoma cells.
作者： Masashi Tamaki , Warren McDonald , Rolando F. Del Maestro
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摘要: We have examined the influence of basic fibroblast growth factor (FGF-2) and vascular endothelial (VEGF) on release collagenase type IV activity production extracellular matrix (ECM) molecules using C6 astrocytoma cells in monolayer culture. Collagenase was significantly increased a dose dependent manner low cell density group by treatment with FGF-2 VEGF but decreased fashion high group. These results were corroborated Western blot technique an antibody to gelatinase A. Addition exogenous laminin fibronectin media minimum concentration 0.1 μg/ml. Laminin reached μg/ml only after factors tested. findings indicate that appear two regulatory mechanisms for which are density. In density, respond dominant effect increasing activity. is due it's down regulation released ECM response VEGF. may be crucial understanding coordination tumor-associated angiogenesis malignant glial cells.
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