Use of a cocktail of monoclonal antibodies and human complement in selective killing of acute lymphocytic leukemia cells.

摘要: Autologous remission bone marrow is a potential source of repopulative stem cells after ablative chemoradiotherapy tumor patients. Even with marrow, one major obstacle to use autologous bone-marrow support the danger reinfusing viable cells. This report describes purging protocol human complement, which seems suitable for eliminating acute lymphatic leukemia (ALL) common ALL type. Lysis blasts induced cocktail 3 monoclonal antibodies IgM type (termed VIB-pool). These are directed against CALLA (CD 10) antigen (VIL-AI antibody) and 2 different epitopes CD24 surface structure (VIB-C5 VIB-E3 antibodies). The efficiency was evaluated leukemic cell lines (Reh-6 Nalm-6) blast from Optimal lysis obtained antibody serum concentrations as low 1 microgram/ml 7% respectively. As standard we propose 20-min incubation at room temperature followed by two 30-min incubations 37 degrees C 25% complement. In dye exclusion test 99% in clonogenic assays detecting elimination up 5 logs 99.99% (= 4 logs) were achieved. Treatment VIB-pool complement had no negative effect on growth normal hemopoietic progenitor CFU-GM, CFU-E BFU-E.