Antimicrobial response of galectin-1 from rock bream Oplegnathus fasciatus: Molecular, transcriptional, and biological characterization.
作者： William Shanthakumar Thulasitha , Navaneethaiyer Umasuthan , Ilson Whang , Bo-Hye Nam , Jehee Lee
DOI: 10.1016/J.FSI.2016.01.010
关键词:
摘要: In this study, we describe the identification and characterization of a proto type galectin, galectin-1, from rock bream Oplegnathus fasciatus (OfGal-1). Galectins are evolutionarily conserved carbohydrate binding lectins that show wide range functions related to development immune physiology. They have been identified as pattern recognition receptors innate system recognize broad microbes. OfGal-1 cDNA comprised 993 bp with an open reading frame 408 encodes 135 amino acids. A single domain was present in acid sequence. The sequence comparison by multiple pairwise alignments phylogenetic tree emphasized strong evolutionary conservation Gal-1. typical β-sandwich structure predicted tertiary structure. constitutive expression mRNA transcripts detected tissues examined, highest heart. Immune challenges live bacteria (Edwardsiella tarda Streptococcus iniae), irido virus, mitogens (lipopolysaccharide poly I:C) modulated mRNAs gills, head kidney, liver. recombinant (rOfGal-1) strongly agglutinatinated human erythrocytes, hemagglutination inhibited lactose D-galactose. (S. iniae, S. parauberis, Escherichia coli, Edwardsiella tarda, Vibrio anguillarum, harveyi, tapetis) ciliate (Miamiensis avidus) were also effectively recognized rOfGal-1. Significant antiviral activity against virus demonstrated Collectively, results study indicate can microbes is vital receptor bream.
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