High Occurrence Rate of Tetracycline (TC)-Resistant Bacteria and TC Resistance Genes Relates to Microbial Diversity in Sediment of Mekong River Main Waterway

作者: Satoru Suzuki , Takeshi Kobayashi , Fujiyo Suehiro , Bui Cach Tuyen , Touch Seang Tana

DOI: 10.1264/JSME2.23.149

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摘要: Spatial monitoring of tetracycline (TC)-resistant bacteria in sediments the Mekong River watershed revealed that main waterway showed a high occurrence rate TC-resistant bacteria, whereas Tonle Sap Lake and Sai Gon estuary did not. The Shannon index (H'), an indicator ecological diversity, was calculated from denaturing gradient gel electrophoresis (DGGE) profiles, which indicated had microbial diversity (high H') compared to estuary; this positively correlated with bacteria. Analysis ribosomal protection protein (RPP) genes tet(M), tet(S) tet(W) same area also RPP genes, suggesting are well conserved across various bacterial species. Further evidence different genotypes tet(M) suggests drug resistance likely have origins, mixed sediment. Sediments therefore potential reservoirs genes.

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