Cyclooxygenase 2 expression in Barrett's esophagus and adenocarcinoma: Ex vivo induction by bile salts and acid exposure
作者： Vivian N. Shirvani , Rodica Ouatu-Lascar , Baljeet S. Kaur , M.Bishr Omary , George Triadafilopoulos
DOI: 10.1016/S0016-5085(00)70254-X
关键词:
摘要: Abstract Background & Aims: Barrett's esophagus (BE) results from chronic, severe gastroesophageal reflux and predisposes to esophageal adenocarcinoma. Cyclooxygenase (COX)-2 is involved in chronic inflammation epithelial cell growth. We investigated COX-2 expression BE adenocarcinoma explore a potential relation between metaplasia or carcinogenesis. Methods: Endoscopic mucosal biopsy specimens of intestinal (n = 30), dysplasia 11), 5) were compared with normal 46) duodenum analyzed by Western blotting immunohistochemistry. Results: Immunoblots revealed constitutive duodenum. protein was significantly higher patients metaplasia, dysplasia, squamous columnar duodenal epithelia heterogenous different regions the surface. Immunohistochemistry prominent staining glands BE, faint basal layers surface In response pulses acid bile salts an ex vivo organ culture system, increased tissues, this effect attenuated selective inhibitor NS-398. Conclusions: The show esophagus, which increases regulated exposure salts. GASTROENTEROLOGY 2000;118:487-496
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