Characterization of insulin-stimulated microtubule-associated protein kinase. Rapid isolation and stabilization of a novel serine/threonine kinase from 3T3-L1 cells.
作者： L B Ray , T W Sturgill
DOI: 10.1016/S0021-9258(18)37813-X
关键词:
摘要: A protein kinase, termed microtubule-associated (MAP) which phosphorylates 2 (MAP-2) in vitro and is stimulated 1.5-3-fold extracts from insulin-treated 3T3-L1 cells has been identified (Ray, L.B., Sturgill, T.W. (1987) Proc. Natl. Acad. Sci. U.S.A. 84, 1502-1506). Here, we describe chromatographic properties of MAP kinase provide biochemical characterization the partially purified enzyme. Isolation enzyme facilitated by its unusually high affinity for hydrophobic interaction chromatography matrices. The molecular weight was determined to be 35,000 gel filtration 37,000 glycerol gradient centrifugation. activity fractions correlated precisely with presence a 40-kDa phosphoprotein detected sodium dodecyl sulfate-polyacrylamide electrophoresis. Km 7 microM ATP does not utilize GTP. Acetyl-CoA carboxylase, citrate-lyase, casein, histones, phosvitin, protamine, ribosomal S6 were all poor substrates relative MAP-2. inhibited fluoride beta-glycerol phosphate but heparin. These distinguish it kinases previously described literature.
sci-hub.st 参考文章(32)
C V Glover, E R Shelton, D L Brutlag, Purification and characterization of a type II casein kinase from Drosophila melanogaster. Journal of Biological Chemistry. ,vol. 258, pp. 3258- 3265 ,(1983) , 10.1016/S0021-9258(18)32856-4
D. Stefanovic, E. Erikson, L.J. Pike, J.L. Maller, Activation of a ribosomal protein S6 protein kinase in Xenopus oocytes by insulin and insulin-receptor kinase. The EMBO Journal. ,vol. 5, pp. 157- 160 ,(1986) , 10.1002/J.1460-2075.1986.TB04190.X
J Blenis, C J Kuo, R L Erikson, Identification of a ribosomal protein S6 kinase regulated by transformation and growth-promoting stimuli. Journal of Biological Chemistry. ,vol. 262, pp. 14373- 14376 ,(1987) , 10.1016/S0021-9258(18)47802-7
M H Cobb, O M Rosen, Description of a protein kinase derived from insulin-treated 3T3-L1 cells that catalyzes the phosphorylation of ribosomal protein S6 and casein. Journal of Biological Chemistry. ,vol. 258, pp. 12472- 12481 ,(1983) , 10.1016/S0021-9258(17)44200-1
Y. Takai, A. Kishimoto, M. Inoue, Y. Nishizuka, Studies on a cyclic nucleotide-independent protein kinase and its proenzyme in mammalian tissues. I. Purification and characterization of an active enzyme from bovine cerebellum. Journal of Biological Chemistry. ,vol. 252, pp. 7603- 7609 ,(1977) , 10.1016/S0021-9258(17)41009-X
M Hoshi, E Nishida, H Sakai, Activation of a Ca2+-inhibitable protein kinase that phosphorylates microtubule-associated protein 2 in vitro by growth factors, phorbol esters, and serum in quiescent cultured human fibroblasts. Journal of Biological Chemistry. ,vol. 263, pp. 5396- 5401 ,(1988) , 10.1016/S0021-9258(18)60730-6
A. Hirsch, O. M. Rosen, Charles S. Rubin, C. Fung, Development of hormone receptors and hormonal responsiveness in vitro. Insulin receptors and insulin sensitivity in the preadipocyte and adipocyte forms of 3T3-L1 cells. Journal of Biological Chemistry. ,vol. 253, pp. 7570- 7578 ,(1978) , 10.1016/S0021-9258(17)34541-6
M H Cobb, An insulin-stimulated ribosomal protein S6 kinase in 3T3-L1 cells. Journal of Biological Chemistry. ,vol. 261, pp. 12994- 12999 ,(1986) , 10.1016/S0021-9258(18)69260-9
E Erikson, J L Maller, Purification and characterization of a protein kinase from Xenopus eggs highly specific for ribosomal protein S6 Journal of Biological Chemistry. ,vol. 261, pp. 350- 355 ,(1986) , 10.1016/S0021-9258(17)42478-1
M I Gonzatti-Haces, J A Traugh, Ca2+-independent activation of protease-activated kinase II by phospholipids/diolein and comparison with the Ca2+/phospholipid-dependent protein kinase. Journal of Biological Chemistry. ,vol. 261, pp. 15266- 15272 ,(1986) , 10.1016/S0021-9258(18)66862-0