Enzymatic Synthesis of Sequence-Defined Synthetic Nucleic Acid Polymers with Diverse Functional Groups.
作者： Dehui Kong , Yi Lei , Wayland Yeung , Ryan Hili
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摘要: The development and in-depth analysis of T4 DNA ligase-catalyzed templated oligonucleotide polymerization toward the generation diversely functionalized nucleic acid polymers is described. NNNNT codon set enables low bias, high fidelity, efficiency for ANNNN libraries comprising various functional groups. robustness method was highlighted in copolymerization a 256-membered library 16 sub-libraries modified with different This enabled synthetic polymer 93.8 % fidelity. process should find ready application nanotechnology, computing, in vitro evolution polymers.
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